{"id":511,"date":"2016-11-19T21:29:28","date_gmt":"2016-11-20T02:29:28","guid":{"rendered":"https:\/\/agaaga1.wordpress.com\/?p=511"},"modified":"2016-11-19T21:29:28","modified_gmt":"2016-11-20T02:29:28","slug":"who-did-it","status":"publish","type":"post","link":"https:\/\/agaaga.khoolbrew.ca\/index.php\/2016\/11\/19\/who-did-it\/","title":{"rendered":"Who did it?"},"content":{"rendered":"<p>Bank robbery. DNA material collected from the crime scene. Two suspects. Who did it?<\/p>\n<p>Lara learned about DNA Profiling and Gel Electrophoresis today through the Canadian Association for Girls in Science (<a href=\"http:\/\/cagis.ca\" target=\"_blank\" rel=\"noopener noreferrer\">cagis.ca<\/a>)<\/p>\n<p>Prepping the micropipette:<\/p>\n<p><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-522\" src=\"https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1950.jpg?resize=1032%2C774&#038;ssl=1\" alt=\"img_1950\" width=\"1032\" height=\"774\" srcset=\"https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1950.jpg?w=2016&amp;ssl=1 2016w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1950.jpg?resize=300%2C225&amp;ssl=1 300w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1950.jpg?resize=1024%2C768&amp;ssl=1 1024w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1950.jpg?resize=768%2C576&amp;ssl=1 768w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1950.jpg?resize=1536%2C1152&amp;ssl=1 1536w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1950.jpg?resize=1200%2C900&amp;ssl=1 1200w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1950.jpg?resize=1980%2C1485&amp;ssl=1 1980w\" sizes=\"auto, (max-width: 1032px) 100vw, 1032px\" \/><\/p>\n<p>Obtain DNA from Suspect 1:<\/p>\n<p><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-526\" src=\"https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1953.jpg?resize=1032%2C774&#038;ssl=1\" alt=\"img_1953\" width=\"1032\" height=\"774\" srcset=\"https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1953.jpg?w=2016&amp;ssl=1 2016w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1953.jpg?resize=300%2C225&amp;ssl=1 300w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1953.jpg?resize=1024%2C768&amp;ssl=1 1024w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1953.jpg?resize=768%2C576&amp;ssl=1 768w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1953.jpg?resize=1536%2C1152&amp;ssl=1 1536w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1953.jpg?resize=1200%2C900&amp;ssl=1 1200w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1953.jpg?resize=1980%2C1485&amp;ssl=1 1980w\" sizes=\"auto, (max-width: 1032px) 100vw, 1032px\" \/><\/p>\n<p>Place DNA into gel electrophoresis:<img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-519\" src=\"https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1966.jpg?resize=1032%2C774&#038;ssl=1\" alt=\"img_1966\" width=\"1032\" height=\"774\" srcset=\"https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1966.jpg?w=2016&amp;ssl=1 2016w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1966.jpg?resize=300%2C225&amp;ssl=1 300w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1966.jpg?resize=1024%2C768&amp;ssl=1 1024w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1966.jpg?resize=768%2C576&amp;ssl=1 768w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1966.jpg?resize=1536%2C1152&amp;ssl=1 1536w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1966.jpg?resize=1200%2C900&amp;ssl=1 1200w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1966.jpg?resize=1980%2C1485&amp;ssl=1 1980w\" sizes=\"auto, (max-width: 1032px) 100vw, 1032px\" \/><\/p>\n<p>Obtain DNA from Suspect 2 and place in gel electrophoresis. Forensic scientist placed DNA from crime scene.<\/p>\n<div class=\"tiled-gallery type-rectangular tiled-gallery-unresized\" data-original-width=\"1032\" data-carousel-extra='null' itemscope itemtype=\"http:\/\/schema.org\/ImageGallery\" > <div class=\"gallery-row\" style=\"width: 1032px; height: 388px;\" data-original-width=\"1032\" data-original-height=\"388\" > <div class=\"gallery-group images-1\" style=\"width: 516px; height: 388px;\" data-original-width=\"516\" data-original-height=\"388\" > <div class=\"tiled-gallery-item tiled-gallery-item-large\" itemprop=\"associatedMedia\" itemscope itemtype=\"http:\/\/schema.org\/ImageObject\"> <a href=\"https:\/\/agaaga.khoolbrew.ca\/index.php\/2016\/11\/19\/who-did-it\/img_1964\/\" border=\"0\" itemprop=\"url\"> <meta itemprop=\"width\" content=\"512\"> <meta itemprop=\"height\" content=\"384\"> <img decoding=\"async\" class=\"\" data-attachment-id=\"518\" data-orig-file=\"https:\/\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1964.jpg\" data-orig-size=\"2016,1512\" data-comments-opened=\"1\" data-image-meta=\"{&quot;aperture&quot;:&quot;2.2&quot;,&quot;credit&quot;:&quot;&quot;,&quot;camera&quot;:&quot;iPhone 6s&quot;,&quot;caption&quot;:&quot;&quot;,&quot;created_timestamp&quot;:&quot;1479569564&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;4.15&quot;,&quot;iso&quot;:&quot;100&quot;,&quot;shutter_speed&quot;:&quot;0.033333333333333&quot;,&quot;title&quot;:&quot;&quot;,&quot;orientation&quot;:&quot;1&quot;}\" data-image-title=\"img_1964\" data-image-description=\"\" data-medium-file=\"https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1964.jpg?fit=300%2C225&#038;ssl=1\" data-large-file=\"https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1964.jpg?fit=1024%2C768&#038;ssl=1\" src=\"https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1964.jpg?w=512&#038;h=384&#038;ssl=1\" srcset=\"https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1964.jpg?w=2016&amp;ssl=1 2016w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1964.jpg?resize=300%2C225&amp;ssl=1 300w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1964.jpg?resize=1024%2C768&amp;ssl=1 1024w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1964.jpg?resize=768%2C576&amp;ssl=1 768w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1964.jpg?resize=1536%2C1152&amp;ssl=1 1536w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1964.jpg?resize=1200%2C900&amp;ssl=1 1200w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1964.jpg?resize=1980%2C1485&amp;ssl=1 1980w\" width=\"512\" height=\"384\" loading=\"lazy\" data-original-width=\"512\" data-original-height=\"384\" itemprop=\"http:\/\/schema.org\/image\" title=\"img_1964\" alt=\"img_1964\" style=\"width: 512px; height: 384px;\" \/> <\/a> <\/div> <\/div> <!-- close group --> <div class=\"gallery-group images-1\" style=\"width: 516px; height: 388px;\" data-original-width=\"516\" data-original-height=\"388\" > <div class=\"tiled-gallery-item tiled-gallery-item-large\" itemprop=\"associatedMedia\" itemscope itemtype=\"http:\/\/schema.org\/ImageObject\"> <a href=\"https:\/\/agaaga.khoolbrew.ca\/index.php\/2016\/11\/19\/who-did-it\/img_1957\/\" border=\"0\" itemprop=\"url\"> <meta itemprop=\"width\" content=\"512\"> <meta itemprop=\"height\" content=\"384\"> <img decoding=\"async\" class=\"\" data-attachment-id=\"517\" data-orig-file=\"https:\/\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1957.jpg\" data-orig-size=\"2016,1512\" data-comments-opened=\"1\" data-image-meta=\"{&quot;aperture&quot;:&quot;2.2&quot;,&quot;credit&quot;:&quot;&quot;,&quot;camera&quot;:&quot;iPhone 6s&quot;,&quot;caption&quot;:&quot;&quot;,&quot;created_timestamp&quot;:&quot;1479569423&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;4.15&quot;,&quot;iso&quot;:&quot;160&quot;,&quot;shutter_speed&quot;:&quot;0.033333333333333&quot;,&quot;title&quot;:&quot;&quot;,&quot;orientation&quot;:&quot;1&quot;}\" data-image-title=\"img_1957\" data-image-description=\"\" data-medium-file=\"https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1957.jpg?fit=300%2C225&#038;ssl=1\" data-large-file=\"https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1957.jpg?fit=1024%2C768&#038;ssl=1\" src=\"https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1957.jpg?w=512&#038;h=384&#038;ssl=1\" srcset=\"https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1957.jpg?w=2016&amp;ssl=1 2016w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1957.jpg?resize=300%2C225&amp;ssl=1 300w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1957.jpg?resize=1024%2C768&amp;ssl=1 1024w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1957.jpg?resize=768%2C576&amp;ssl=1 768w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1957.jpg?resize=1536%2C1152&amp;ssl=1 1536w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1957.jpg?resize=1200%2C900&amp;ssl=1 1200w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_1957.jpg?resize=1980%2C1485&amp;ssl=1 1980w\" width=\"512\" height=\"384\" loading=\"lazy\" data-original-width=\"512\" data-original-height=\"384\" itemprop=\"http:\/\/schema.org\/image\" title=\"img_1957\" alt=\"img_1957\" style=\"width: 512px; height: 384px;\" \/> <\/a> <\/div> <\/div> <!-- close group --> <\/div> <!-- close row --> <\/div>\n<p>Plug it in, wait 10 minutes, and voila! Suspect 1 gets nailed.\u00a0You can see the bands if you zoom.<\/p>\n<p><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-521\" src=\"https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_19701.jpg?resize=1032%2C1376&#038;ssl=1\" alt=\"img_1970\" width=\"1032\" height=\"1376\" srcset=\"https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_19701.jpg?w=1512&amp;ssl=1 1512w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_19701.jpg?resize=225%2C300&amp;ssl=1 225w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_19701.jpg?resize=768%2C1024&amp;ssl=1 768w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_19701.jpg?resize=1152%2C1536&amp;ssl=1 1152w, https:\/\/i0.wp.com\/agaaga.khoolbrew.ca\/wp-content\/uploads\/2016\/11\/img_19701.jpg?resize=1200%2C1600&amp;ssl=1 1200w\" sizes=\"auto, (max-width: 1032px) 100vw, 1032px\" \/><\/p>\n<p>Way more high tech than when LL did this 20 odd years ago in the Medical Microbiology and Infections Diseases lab at the U of A!<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Bank robbery. DNA material collected from the crime scene. Two suspects. Who did it? Lara learned about DNA Profiling and Gel Electrophoresis today through the Canadian Association for Girls in Science (cagis.ca) Prepping the micropipette: Obtain DNA from Suspect 1: Place DNA into gel electrophoresis: Obtain DNA from Suspect 2 and place in gel electrophoresis. [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[1],"tags":[11,23],"class_list":["post-511","post","type-post","status-publish","format-standard","hentry","category-uncategorized","tag-cagis","tag-lara"],"jetpack_featured_media_url":"","jetpack-related-posts":[],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/agaaga.khoolbrew.ca\/index.php\/wp-json\/wp\/v2\/posts\/511","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/agaaga.khoolbrew.ca\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/agaaga.khoolbrew.ca\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/agaaga.khoolbrew.ca\/index.php\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/agaaga.khoolbrew.ca\/index.php\/wp-json\/wp\/v2\/comments?post=511"}],"version-history":[{"count":0,"href":"https:\/\/agaaga.khoolbrew.ca\/index.php\/wp-json\/wp\/v2\/posts\/511\/revisions"}],"wp:attachment":[{"href":"https:\/\/agaaga.khoolbrew.ca\/index.php\/wp-json\/wp\/v2\/media?parent=511"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/agaaga.khoolbrew.ca\/index.php\/wp-json\/wp\/v2\/categories?post=511"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/agaaga.khoolbrew.ca\/index.php\/wp-json\/wp\/v2\/tags?post=511"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}